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Rabbit Human Copeptin  Antibodies (Vasopressin-Associated Glycopeptide, VAG), BORIS Y.

Rabbit Human Copeptin Antibodies (BORIS Y)
Sizes

This is an antibody preparation generated in rabbit by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel School of Medicine at Dartmouth College) using a synthetic peptide representing the last 18 C-terminal residues of the 39 amino acid glycopeptide, coupled to bovine thyroglobulin as antigen.

Specificity: No measureable reactivity with rat or mouse copeptin, no measureable reactivity with vasopressin or human vasopressin-related neurophysin.

Tested applications                          IHC-formaldehyde-fixed tissues, Flow Cytometry, RIA, ELISA

Properties

Form                                                Liquid

Storage Instructions                        Shipped at 4⁰C. Store at 4⁰C short term (1-2 weeks). Upon delivery aliquot and store at -20⁰C or -80⁰C. Avoid freeze/Thaw cycling.

Storage buffer                                 Preservative none.  Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20⁰C.

                                                        Constituents: Whole serum

Purity                                               Whole Antiserum

Research Areas:                             Neuroscience, Endocrinology, Neuropeptides, Hormones, Neuroendocrinology, Measurement of Oxytocin Release.

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Woomera Therapeutics Inc.  guarantees use of BORIS Y in each of the tested applications:

The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user.

Application

IHC                                                   1/1000-1/2000 Use at an assay dependent concentration

RIA                                                   1/15,000-20,000, Sensitivity to 2 pg; sensitivity  range, 2-320 pg; 50% displacement at 40 pg; <no measurable cross-reaction with AVP or VP-HNP.

Flow Cytometry                                 >1/1000

Western                                              >1/1000

References

Friedmann, A.S., Malott, K.A., Memoli, V.A., Pai, S.I., Yu, X.-M., and North, W.G.  Products of vasopressin gene expression in small-cell carcinoma of the lung.  Br. J. Cancer  69:260-263, 1994.

North, W.G., Pai, S., Friedmann, A., Yu, X., Fay, M., and Memoli, V.  Vasopressin gene related products are markers of human breast cancer.  Breast Cancer Research and Treatment 34:229-235, 1995.


Product Code                     BORIS Y