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These are antibody preparations generated in rabbit by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel School of Medicine at Dartmouth College) using vasopressin associated human neurophysin protein purified by preparative electrophoresis and preparative isofocusing  as antigen.

Specificity                                     1-5% cross-reactivity with Human Neurophysins 3 (Vasopressin-Associated Human Neurophysin, VP-HNP).

Tested Applications                       IHC-formaldehyde fixed tissues, Flow cytometry , RIA, ELISA, Western

Properties

Form                                             Liquid

Storage Instructions                     Shipped at 4⁰C. Store at 4⁰C short term (1-2 weeks). Upon delivery aliquot and store at -20⁰C or -80⁰C. Avoid freeze/Thaw cycling.


Storage buffer                              Preservative none. Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20⁰C.
                                                     Constituents: Whole serum

Purity                                            Whole Antiserum

Clonality                                       Polyclonal

Isotype                                          IgG, Behaves chromatographically like mouse IgG2B

Research Areas                            Neuroscience, Endocrinology, Neuropeptides, Hormones, Oncology, Neuroendocrinology, Measurement of Vasopressin Release.

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Woomera Therapeutics Inc.  guarantees use of ARC, PTR, and PL in each of the tested applications:

The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user.

Application

IHC                                                 1:5000-10,000, Use at an assay dependent concentration

RIA                                                 1/10,000-1/50,000, Sensitivity to 5-10 pg, range 10-320 pg; 50% displacement at ~50 pg; ; ~1-5% OT-HNP cross-reaction.

ELISA                                             1/10,000

Flow Cytometry                              1/1000

Western                                           1/1000

Reference

North, W.G., LaRochelle, F.T., Jr., Melton, J., and Mills, R.C.  Isolation and partial characterization of two human neurophysins: Their use in the development of specific radioimmunoassays.  J. Clin. Endocrinol. Metab. 51:884-891, 1980.

North, W.G., Maurer, L.H., Valtin, H., and O'Donnell, J.F.  Human neurophysins as potential tumor markers for small cell carcinoma of the lung:  Application of specific radioimmunoassays.  J. Clin. Endocrinol. Metab.  51:892-896, 1980.

North, W.G., Ware, J., Chahinian, A.P., Perry, M., O'Donnell, J., and Maurer, L.H.  Clinical evaluation of the neurophysins as tumor markers in small cell lung cancer.  Recent Results in Cancer Res. 99:187-193, 1985.

North, W.G., Ware, J., Maurer, L.H., Chahinian, A.P., and Perry, M.  Neurophysins as tumor markers for small cell carcinoma of the lung: A cancer and leukemia group B evaluation.  Cancer 62:1343-1347, 1988.

North, W.G., Hirsh, V., Lisbona, R., Schulz, J., and Cooper, B.  Imaging of small cell carcinoma using 131I-labelled antibodies to vasopressin associated human neurophysin (VP-HNP). Nuclear Medicine Communications 10:643-651, 1989.

North, W.G., Harbaugh, R., and Reeder, T.  An evaluation of human neurophysin production in Alzheimer's disease:  Preliminary observations.  Neurobiol. Aging  13(2):261-265, 1992.

North, W.G.  Neuropeptide production by small-cell carcinoma:  Vasopressin and oxytocin as plasma markers of disease.  J. Clin. Endocrin. Metab. 73:1316-1320, 1991.

Friedmann, A.S., Memoli, V.A., Yu, X-M., and North, W.G.  Biosynthesis of vasopressin by gastrointestinal cells of Brattleboro and Long-Evans rats.  Peptides 14:607-612, 1993.

Friedmann, A.S., Memoli, V.A., and North, W.G.  Vasopressin and oxytocin production by non-neuroendocrine lung carcinomas: an apparent low incidence of gene expression.  Cancer Letters 75:79-85, 1993.

Friedmann, A.S., Malott, K.A., Memoli, V.A., Pai, S.I., Yu, X.-M., and North, W.G.  Products of vasopressin gene expression in small-cell carcinoma of the lung.  Br. J. Cancer  69:260-263, 1994.

North, W.G., Pai, S., Friedmann, A., Yu, X., Fay, M., and Memoli, V.  Vasopressin gene related products are markers of human breast cancer.  Breast Cancer Research and Treatment 34:229-235, 1995.

Friedmann, A.S., Fay, M.J., Memoli, V.A. and North, W.G.  Factors regulating the production of vasopressin-associated human neurophysin by small-cell carcinoma of the lung:  Evaluation by computer-enhanced quantitative immunocytochemistry.  Neuropeptides 28:183-189, 1995.

Fay, M.J., Mathew, R.S., Donnelly, E.M., Memoli, V.A., and North, W.G.  Immunohistochemical evaluation of vasopressin gene expression in fibrocystic breast disease.   Endocrine Pathology, In Press, 2000.

North,W.G., Wells,W., Fay, M.J., Mathew, R.J., Donnelly, E.M., and Memoli, V.A. Immunohistochemical evaluation of vasopressin  expression in breast fibrocystic disease and ductal carcinoma in situ (DCIS). Endocrin. Pathology, 14, 257-262, 2003.

 

Product codes                     ARC


Neurophysin  Antibodies  (OT-HNP, HNP1) Antibodies  (ARC)

Neurophysin Antibodies (ARC)
Sizes