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These are antibody preparations generated in rabbit by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel School of Medicine at Dartmouth College) using oxytocin-associated bovine neurophysin protein purified by preparative electrophoresis as antigen.

Specificity:  Less than 1% reactivity with Bovine Neurophysin2 (Vasopressin-Associated Bovine Neurophysin, VP-BNP) in RIA

Tested Applications                         IHC-formaldehyde fixed tissues, Flow cytometry , RIA, ELISA

Properties

Form                                               Liquid

Storage Instructions                      Shipped at 4⁰C. Store at 4o C short term (1-2 weeks). Upon delivery aliquot and store at -20⁰C or -80⁰C. Avoid freeze/Thaw cycling.

Storage buffer                                Preservative none.  Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20⁰C.

                                                       Constituents: Whole serum

Purity                                             Whole Antiserum

Clonality                                        Polyclonal
 
Isotype                                           IgG, Behaves chromatographically like mouse IgG2B

Research Areas                             Neuroscience, Endocrinology, Neuropeptides, Hormones, Neuroendocrinology, Measurement of Oxytocin Release.

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Woomera Therapeutics Inc.  guarantees use of FPY, CRS, DORGHT in each of the tested applications:

The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user.

Application

IHC                                                  Use at an assay dependent concentration

RIA                                                  1/20,000; Sensitivity to 20 pg; range of sensitivity 20-1280 pg; < 1% VP-BNP cross-reaction.

Western                                           >1/1000


Rabbit Oxytocin Bovine (cow) Neurophysin Antibodies (OT-BNP)
Sizes

Rabbit Oxytocin Bovine (cow)  Neurophysin  Antibodies (BNP1, OT-BNP) ,(FLOPSY, DORIGHT, CRUSADER)