Rabbit Arginine-Vasopressin Antibodies (FZE)
Sizes


This is an antibody preparation generated in rabbit by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel  Medical  School at Dartmouth) using arginine-vasopressin coupled to bovine thyroglobulin.

Specificity                                         Less than 0.1% cross-reactivity with oxytocin,  in RIA

Tested Applications                          IHC-formaldehyde fixed tissues, Flow cytometry, , RIA, ELISA

Properties

Form                                                Liquid

Storage Instructions                     Shipped at 4⁰C. Store at 4⁰C short term (1-2 weeks). Upon delivery aliquot and store at -20⁰C or -80⁰C. Avoid    freeze/Thaw cycling.

Storage buffer                                Preservative none.  Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20⁰C.

                                                      Constituents: Whole serum

Purity                                              Whole Antiserum

Clonality                                         Polyclonal

Isotype                                            IgG, Behaves chromatographically like mouse IgG2B

Research Areas:                             Neuroscience, Endocrinology, Neuropeptides, Hormones, Neuroendocrinology, OBGYN, Cancer

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Woomera Therapeutics Inc.  guarantees use of FZE in each of the tested applications:

The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user.

Application

IHC                                                   Use at an assay dependent concentration

RIA                                                  1/30,000-99,000. Sensitivity to 0.4 pg/ml; 50% displacement ~5 pg, range 0.4-32 pg; <0.1% OT cross-reaction.

ELISA                                              1/30,000-50,000

Flow Cytometry                               1/1000-5000

Western                                            1/1000-5000

References

North, W.G.  Neuropeptide production by small-cell carcinoma:  Vasopressin and oxytocin as plasma markers of disease.  J. Clin. Endocrin. Metab. 73:1316-1320, 1991.

Cheng, S.W.T., and North, W.G.  Absence of negative feedback by oxytocin on release from magnocellular neurones in conscious rats.  Can. J. Physiol. Pharmacol.  70(1):100-105, 1992.

Piqueux, C., Hagelstein M.T., Keegan, B., Legros, V., and North, W.G. Vasopressin and Oxytocin mitogen transduction pathway in small cell lung cancer cells.  Endocr.-Relat. Cancer, 4:871-875, 2004.

 
Product Code                                                    FZE


Rabbit Arginine-Vasopressin  Antibodies (FOZZIE, FZE)

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