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|Rabbit Oxytocin Antibodies (BRD)|
|Rabbit Vasopressin-Rat / Mouse Neurophysin Antibodies (VP-RNP, RNP1) Antibodies (HK 76)|
|Rabbit Oxytocin-Rat / Mouse Neurophysin Antibodies , PIPPIN (PP76); FRODO (FD 76)|
|Rabbit Human Copeptin Antibodies (Vasopressin-Associated Glycopeptide, VAG), (BORIS Y)|
|Rabbit Oxytocin Bovine (cow) Neurophysin Antibodies (OT-BNP)|
These are antibody preparations generated in rabbits by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel School of Medicine at Dartmouth College) using oxytocin-associated rat neurophysin (OT-RNP, RNP2) protein (PP 76), and oxytocin-associated rat neurophysin derived (OT-RNP’, RNP3) protein (FD 76) protein, each purified by preparative electrophoresis as antigen.
Specificity: Less than 1% reactivity with Rat and Mouse Neurophysins 1(Vasopressin Associated Rat and Mouse Neurophysin), and 100% cross-reaction with Mouse Oxytocin- Related Neurophysin.
Tested Applications IHC-formaldehyde fixed tissues, Flow cytometry , RIA, ELISA
Storage Instructions Shipped at 4⁰C. Store at 4⁰C short term (1-2 weeks). Upon delivery aliquot and store at -20⁰C or -80⁰C. Avoid freeze/Thaw cycling.
Storage buffer Preservative none. Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20⁰C.
Constituents: Whole serum
Purity Whole Antiserum
ype IgG, Behaves chromatographically like mouse IgG2B
Research Areas: Neuroscience, Endocrinology, Neuropeptides, Hormones, Neuroendocrinology, Measurement of Vasopressin Release.
Woomera Therapeutics Inc. guarantees use of PP in each of the tested applications:
The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user.
IHC 1/5000-1/10000, Use at an assay dependent concentration
RIA 1/20,000-1/30,000, Sensitivity to 20 pg; range of measurement 20-1280 pg, 50% displacement at 160 pg or 80 pg; <1% VP-RNP cross-reaction, PP 76 does not distinguish between R(M)NP2 and 3; FD 76 shows some preference for R(M)NP3.
Flow Cytometry 1/1000
North, W.G., and Valtin, H. The purification of rat neurophysins by a method of preparative polyacrylamide gel electrophoresis. Analyt. Biochem. 78:436-450, 1977.
North, W.G., LaRochelle, F.T., Jr., and Hardy, G.R. Radioimmunoassays for individual rat neurophysins. J. Endocrinol. 96:373-386, 1983.
North, W.G., and Mitchell, T.I. Evolution of neurophysin proteins: Partial amino acid sequences of rat neurophysins. FEBS Letters 126:41-44, 1981.
Fisher, A.W.F., Gill, V., Wong, K., Raghatvan, S., North, W., and Lederis, K. Anatomical and developmental aspects of the neurons terminating in the posterior pituitary. In: Neurosecretion: Molecules, Cells, Systems. D.S. Farner and K. Lederis (eds.) Plenum Publishing Corp. pp. 39-47, 1982.
Wong, K.W., Fisher, A., Lederis, K., and North, W. The ontogeny of the oxytocin/neurophysin system of the Brattleboro rat. Ann. N.Y. Acad. Sci. 394:177-182, 1982.
North, W.G., Gellai, M., and Hardy, G. Oxytocin and oxytocin-associated neurophysin evaluation by RIA in the Brattleboro rat: Turnover. Ann. N.Y. Acad. Sci. 394:167-172, 1982.
Cheng, S.W.T., and North, W.G. Effects of acute increases in plasma osmolality on plasma vasopressin-associated neurophysin in conscious rats: implications for osmoregulation. Neuroendocrin. 40:363-368, 1985.
Cheng, S.W.T., and North, W.G. Function of vasopressinergic neurons in rats under conscious and anesthetized conditions. Am. J. Physiol. 248:E155-E161, 1985.
Stern, J.E., Mitchell, T., Herzberg, V.L., and North, W.G. Secretion of vasopressin, oxytocin and two neurophysins from rat hypothalamo-neurohypophyseal explants in organ culture. Neuroendocrin., 43:252-258, 1986.
Gonzalez, C.B., Caorsi, C.E., North, W.G., and Berrios, O.T. Purification of bovine neurosecretory granule membranes by density gradient centrifugation. Analytical Biochem. 157:316-322, 1986.
O'Connor, E.F., Cheng, S.W.T., and North, W.G. Effects of intraperitoneal injection of lithium chloride on neurohypophyseal activity: implications for behavioral studies. Physiology and Behavior 40:91-95, 1987.
Cheng, S.W.T., and North, W.G. Reduced responsiveness to acute salt-loading of vasopressin-neurons and oxytocin-neurons in the heterozygous Brattleboro rat. Neuroendocrinol. 45:93-98, 1987.
Cheng, S.W.T., and North, W.G. Vasopressin reduces release from vasopressin-neurons and oxytocin-neurons by acting on V2-like receptors. Brain Res. 479:35-39, 1989.
Cheng, S.W.T., O'Connor, E.F., and North, W.G. Influence of opioid peptides on release from vasopressin and oxytocin neurones of the hypothalamoneurohypophyseal system: Effects of naloxone in the conscious rat. Can. J. Physiol. Pharmacol. 68:568-574, 1990.
Cheng, S.W.T., and North, W.G. Influence of pentobarbital and urethane on release from magnocellular neurons. Neuroendocrinology 54:482-487, 1991.
North, W.G., O'Connor, E.F., and Gonzalez, C.B. Single-step isolation and sequencing of vasopressin and oxytocin precursors. Peptides 13:395-400, 1992.
Product Codes: PP76, FD 76