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Rabbit Oxytocin-Rat / Mouse  Neurophysin Antibodies   (Rat Neurophysin 2 and 3, OT-RNP),PIPPIN (PP76); FRODO (FD 76)

Rabbit Oxytocin-Rat / Mouse Neurophysin Antibodies (PP76;FD76)

These are  antibody preparations generated in rabbits by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel School of Medicine at Dartmouth College) using oxytocin-associated rat neurophysin (OT-RNP, RNP2) protein (PP 76), and oxytocin-associated rat neurophysin derived (OT-RNP’, RNP3) protein (FD 76) protein, each  purified by preparative electrophoresis as antigen.

Specificity:  Less than 1% reactivity with Rat and Mouse Neurophysins 1(Vasopressin Associated Rat and Mouse Neurophysin), and 100% cross-reaction with Mouse Oxytocin- Related Neurophysin.

Tested Applications                         IHC-formaldehyde fixed tissues, Flow cytometry , RIA, ELISA


Form                                               Liquid

Storage Instructions                       Shipped at 4⁰C. Store at 4⁰C short term (1-2 weeks). Upon delivery aliquot and store at -20⁰C or -80⁰C. Avoid freeze/Thaw cycling.

Storage buffer                             Preservative none.  Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20⁰C.

                                                       Constituents: Whole serum

Purity                                              Whole Antiserum

Clonality                                          Polyclonal

 ype                                                 IgG, Behaves chromatographically like mouse IgG2B

Research Areas:                             Neuroscience, Endocrinology, Neuropeptides, Hormones, Neuroendocrinology, Measurement of Vasopressin Release.


Woomera Therapeutics Inc.  guarantees use of PP in each of the tested applications:

The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user.


IHC                                                    1/5000-1/10000, Use at an assay dependent concentration

RIA                                                   1/20,000-1/30,000, Sensitivity to 20 pg; range of measurement 20-1280 pg, 50% displacement at 160 pg  or 80 pg; <1% VP-RNP cross-reaction, PP 76 does not distinguish between R(M)NP2 and 3; FD 76 shows some preference for R(M)NP3.

ELISA                                                1/10,000

Flow Cytometry                                 1/1000

Western                                            1/1000


North, W.G., and Valtin, H.  The purification of rat neurophysins by a method of preparative polyacrylamide gel electrophoresis.  Analyt. Biochem. 78:436-450, 1977.

North, W.G., LaRochelle, F.T., Jr., and Hardy, G.R.  Radioimmunoassays for individual rat neurophysins.  J. Endocrinol. 96:373-386, 1983.

North, W.G., and Mitchell, T.I.  Evolution of neurophysin proteins:  Partial amino acid sequences of rat neurophysins.  FEBS Letters 126:41-44, 1981.

Fisher, A.W.F., Gill, V., Wong, K., Raghatvan, S., North, W., and Lederis, K.  Anatomical and developmental aspects of the neurons terminating in the posterior pituitary.  In: Neurosecretion: Molecules, Cells, Systems. D.S. Farner and K. Lederis (eds.) Plenum Publishing Corp. pp. 39-47, 1982.

Wong, K.W., Fisher, A., Lederis, K., and North, W.  The ontogeny of the oxytocin/neurophysin system of the Brattleboro rat.  Ann. N.Y. Acad. Sci. 394:177-182, 1982.

North, W.G., Gellai, M., and Hardy, G.  Oxytocin and oxytocin-associated neurophysin evaluation by RIA in the Brattleboro rat: Turnover.  Ann. N.Y. Acad. Sci. 394:167-172, 1982.

Cheng, S.W.T., and North, W.G.  Effects of acute increases in plasma osmolality on plasma vasopressin-associated neurophysin in conscious rats: implications for osmoregulation.  Neuroendocrin. 40:363-368, 1985.

Cheng, S.W.T., and North, W.G.  Function of vasopressinergic neurons in rats under conscious and anesthetized conditions.  Am. J. Physiol. 248:E155-E161, 1985.

Stern, J.E., Mitchell, T., Herzberg, V.L., and North, W.G.  Secretion of vasopressin, oxytocin and two neurophysins from rat hypothalamo-neurohypophyseal explants in organ culture.  Neuroendocrin., 43:252-258, 1986.

Gonzalez, C.B., Caorsi, C.E., North, W.G., and Berrios, O.T.  Purification of bovine neurosecretory granule membranes by density gradient centrifugation. Analytical Biochem. 157:316-322, 1986.

O'Connor, E.F., Cheng, S.W.T., and North, W.G.  Effects of intraperitoneal injection of lithium chloride on neurohypophyseal activity: implications for behavioral studies.  Physiology and Behavior 40:91-95, 1987.

Cheng, S.W.T., and North, W.G.  Reduced responsiveness to acute salt-loading of vasopressin-neurons and oxytocin-neurons in the heterozygous Brattleboro rat. Neuroendocrinol. 45:93-98, 1987.

Cheng, S.W.T., and North, W.G.  Vasopressin reduces release from vasopressin-neurons and oxytocin-neurons by acting on V2-like receptors. Brain Res. 479:35-39, 1989.

Cheng, S.W.T., O'Connor, E.F., and North, W.G.  Influence of opioid peptides on release from vasopressin and oxytocin neurones of the hypothalamoneurohypophyseal system:  Effects of naloxone in the conscious rat.  Can. J. Physiol. Pharmacol. 68:568-574, 1990.

Cheng, S.W.T., and North, W.G.  Influence of pentobarbital and urethane on release from magnocellular neurons.  Neuroendocrinology  54:482-487, 1991.

North, W.G., O'Connor, E.F., and Gonzalez, C.B.  Single-step isolation and sequencing of vasopressin and oxytocin precursors.  Peptides 13:395-400, 1992.

Product Codes: PP76, FD 76